The concert will raise money for Children In Need and will also be broadcast live on BBC1 as well as BBC Radio 1.
N-Dubz, JLS, James Morrison and Hugh Laurie will all perform at the show, which is being hosted by BBC Radio 1 DJs Chris Moyles and Fearne Cotton as well as actor David Tennant.
Barlow told BBC Radio One: 'I'm excited about this - the last one was great. Everybody was crazy about it. I thought why not - let's do it again. We're moving it out of town.'
The event will take place at the MEN arena in Manchester on November 17, 2011, and 12,000 tickets will go on sale with proceeds going to Children In Need.