The musician, 65, founded rehab clinic The Crossroads Centre in Antigua in 1998 and has held two previous auctions to raise cash for its maintenance.
He said: 'I am very happy that Bonhams have agreed to host my third guitar auction.”
More than 70 guitars and 70 amps will be up for grabs for anyone hoping to own a piece of Clapton’s musical history.
Fans can splash out on his one-of-a-kind black Fender Stratocaster from his 2005 tour, which is expected to sell for around $30,000.
The auction will take place at Bonhams & Butterfields auction house in Los Angeles, on February 17.