Just hours after a video of the 53-year-old mocking a fan's gift with her microphone switched on was leaked online, Madge has now reportedly said less-than-friendly things about the You and I singer.
Gaga, 25, real name Stefani Germanotta, has previously spoken openly about her love for the Material Girl.
But it appears that the pop star's admiration isn't a shared.
Belgian magazine Le Soir have reported that they asked the singer about how she feels about her fans, including Lady Gaga.
She replied: 'I have no comment to make about her obsession towards me because I don't know whether her behaviour is something deep and meaningful, or superficial.'
Madonna's spokesperson Liz Rosenberg has since denied the quote, telling British newspaper the Daily Mail: 'This is not true. Madonna did not say anything negative about Lady Gaga during her press conference.'