The 54-year-old, who is currently promoting new movie Larry Crowne, said he was the driving force behind the decision to end the character's life in the movie.
In an interview on BBC Radio 5 Live's Film Review, Hanks was asked by a caller if there was any discussion about Hooch living.
He said: 'I have to make a confession; I was the main proponent of killing Hooch.
'It was a Disney movie and when we were putting it together I stood up at a table and pounded my fist and said, in the grand Disney tradition of Old Yeller, Hooch must die... and so they killed Hooch.
'We killed Hooch and we never should have. We should have I guess kept that doggy alive, so we wouldn't have made the children cry.'
He added: 'It's my fault, I take full responsibility.'