It comes after it was reported that the 29-year-old and her rapper hubby were having problems.
She told the crowd at the intimate O2 Shepherd's Bush Empire concert on Monday that 'in relationships you can get too comfortable'.
According to British newspaper the Daily Mirror, the Halo hitmaker then looked over at Jay-Z in the crowd and added: 'Sometimes you take people for granted.'
Fresh from wowing the crowds at Glastonbury Festival, the singer kicked off her hour-long set with her current single Run The World.
But before belting out her hit 1 Plus 1 she told Jay-Z: 'Make love to me!'