The 23-year-old singer, who has previously discussed her own bisexuality at length, believes homosexual men and women are "born" that way.
Gaga – who claims her new video 'Telephone' is "groundbreaking" because she showcases of different types of sexuality in the nine-minute promo - said: "There are transsexual women and transgender women and suddenly it becomes poisonous and something else because there are some people in this world that believe being gay is a choice. It's not a choice, we're born this way.
"That's why for me this video is groundbreaking because it has one foot in the art community and one foot in the commercial world."
The 'Paparazzi' singer stars in the video with Beyonce Knowles – who also lends her vocals to the track – and prior to recording Gaga promised Beyonce it would be a classic.
She added in an interview 2Day FM's Kyle and Jackie O radio show: "I told Beyonce this after it aired, you'll see this video is not just great now but six months from now what this video will mean.
"Hearing people say the video is sadomasochistic or that the video promotes murder for young people, it's my personal belief that the video is getting so much attention, not because of those themes because I've done those themes before, haven't I?
"I've done those themes in many of my videos but it's because it's with Beyonce and it's because there are so many homosexual themes."
In the video Gaga kisses a female prison warden, is broken out of jail by Beyonce, kills someone and makes a poisoned sandwich for a group of truckers.
In the past the eccentric pop star has admitted she could easily fall in love with a woman.
She said: "Of course. There are no rules or limits when it comes to love. But I see love as separate from sex."