The 28-year-old former X Factor judge was set to sing with Gary Barlow at the show on November 17 but she has now confirmed she will not perform after recently visiting her doctor.
According to UK newspaper Daily Mirror, Cole's spokesperson said: 'Cheryl Cole has sadly had to pull out of a performance at CIN Rocks this week as she is suffering from pharyngitis.'
There had been reports that Cole was ill and there were doubts about whether or not she would appear and Barlow had spoken about the possibility of her not performing.
He previously said: 'We're still waiting to hear but, if it doesn't happen, I don't know what we're going to do. I might just have to get on the piano and do a bit of Roll Out The Barrel.'