Students at low-income Whitney Elementary were ecstatic when the singer took to the stage to perform songs from his Christmas album.
Two months ago he had pledged to donate $100,000 to the school's 650 student body, and during his visit he handed over the cheque.
He also treated the students, many of whom are homeless, to an additional $100,000 in Christmas gifts.
Superstore Target also matched Bieber's generous pledge, donating an additional $100,000 to the struggling school.
The money will be used to support the children and their families in order to help keep the youths in school.